Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines

Abstract Background An extract of Ficus religiosa latex has been previously found to possess potent pharmacological activity with high antioxidant content phytochemical. The present research was conducted investigate the chemo-preventive efficacy on human breast adenocarcinoma MDA MB 231, neroblastoma IMR 32, and colorectal HCT 116 cell lines. Results results showed that crude induced cytotoxicity in all selected lines IC 50 value 4.8 ± 1.13 μg/ml against 32 line. cycle analysis indicated arrest accumulation cells at G1 phase case 231 whereas G2/M phase. clear bands fragments observed DNA ladder experiments apoptosis is by extracts This could be correlated gene level expression studies pro-apoptotic (p53 caspase-3) anti-apoptotic (Bcl-2, AKT) genes, which got upregulated downregulated, respectively. Conclusion Based experimental evidence, contains phytochemicals antitumor activities.